SPE (Solid Phase Extraction)
What is different between conventional column chromatography and solid phase extraction?
Column chromatography is a very old technique for separation. It uses silica gel, alumina, or other sorbent to separate mixtures. The mode is like the normal phase HPLC. Around 40 years ago, silica gel bonded with C18 is packed into small columns (sometimes called cartridges) and is used to extract organic compounds from water, as an alternative of liquid/liquid partition. This practice is called solid phase extraction. It is actually one type of column chromatography, which is like the reverse phase HPLC. Nowadays, Silica gel, Alumina are also packed into small cartridges and referred to as SPE columns. So now it seems as long as the sorbent is packed into small cartridges, the practice is called solid phase extraction.
What are normal procedures in solid phase extraction?
SPE normally involves 5 steps:
1) activate to make the sorbent wet well;
2) equilibrate to adjust the extraction strength;
3) load sample;
4) remove interference;
5) wash down and collect targeted fraction.
How to select solid phase extraction columns?
SPE columns may be classified, using the practice in HPLC, as reverse phase type, normal phase type, ion exchange type, and mixed mode type. If the sample is aqueous (such as water, urine, plasma, juice), C18 columns (reverse phase type) are most commonly used. If the sample is in organic solvents, a normal phase column (Florisil, Silica gel, Alumina etc.) should be used.
How to select solvents for activation, equilibration and collection in solid phase extraction?
Activation solvent should be able to wet the SPE column sorbent well and compatible with the solvent for equilibration. For columns of reverse phase type, methanol, iso propanol or other polar solvents can be used. For columns of normal phase type, hexane, toluene, or other low polarity solvents can be used. Solvents for equilibration should be compatible with the activation solvent, wet the sorbent well, and of low elution power. If the elution power is high, targeted compound will not be trapped well on the column. For reverse phase column, water or buffer solution is often used for equilibration. For normal phase column, a low polarity solvent is often used for equilibration. Solvents for collection should be compatible with previously used elution solvent, can remove the targeted compounds with small volume and easy to evaporate.
How to decide flow rate in solid phase extraction?
For 3-mL column the flow rate is normally 3-5 mL/min.
For 6-mL column, the flow rate is normally 6-10 mL/min.
If the column is of ion exchange nature, the flow rate is normally at lower end.
For reverse phase column, flow rate ma be set at the higher end.
When do I need to adjust the pH of sample and elution solvent in solid phase extraction?
When the pH can affect extraction of targeted compounds (normally ionizable compounds, such as amines and acids) or the sample matrix that can affect analysis.
Is air bubble a problem in solid phase extraction?
When elution is by gravity, air in the sorbent can change the flow path of solvents and reduce the extraction efficiency. As a standard practice in old type column chromatography, the sorbent must be covered with solvent all the time to avoid air bubble. However in the new SPE practice, the sorbent particle is much smaller and the elution is by a pump or pressure. Air bubble in sorbent can be pushed out quickly. So it is not a problem anymore. Actually the column is often purged with air for solvent exchange.
Do I need to keep the sorbent bed wet all the time during solid phase extraction?
As mentioned in the above question, for SPE using a solvent pump, sorbent bed does need to be wet all the time. For example after a C18 column is activated with methanol, the column can be purged with air and then load an aqueous sample.
When do I need to dry the solid phase extraction column before fraction collection?
When an aqueous sample is loaded or aqueous solvents are used, solvent for collection may have problem to interact with the column properly. Beside the water from column may end up in the collected fraction and affect further evaporation. The SPE column can be dried by purging nitrogen or air. Flow rate of gas cannot be too high or some targeted compounds may be lost. It may be controlled at 300-500 mL/min.
When my recovery result is low in solid phase extraction, what factors should I check?
If the targeted compounds are polar, major cause of low recovery would be the extraction efficiency. Relevant factors are strength of elution solvents, amount of sorbent in the column, volume of sample, and pH of the sample solution.
If the targeted compounds are of low polarity (such as PAH, PCB, hydrocarbon), major causes of low recovery would be adsorption by the container and tubing and evaporation loss. Relevant factors are inertness of the wetting components and the column and compatibility of the solvents with the targeted compounds.
Can I regenerate used solid phase extraction columns for repeated use?
A used SPE column will trap components from sample matrix and particles or large molecules (such as humic acids and proteins) may also block the column. A SPE column may be re used in two cases:
1) the sample matrix is simple so the column capacity is not fully used; or 2) the trapped interference from sample can be removed. In the second case, only C18 column is easy to remove trapped sample matrics. For normal phase column and ion exchange column, removing the trapped components is not easy. In general, if you are using a reverse phase column and your sample is clean, you may regenerate the column for repeated use.
What is Solid Phase Extraction (SPE)?
Solid Phase Extraction is used in the chemical analysis of fluid samples by removing unwanted constituents and keeping only the analytes of interest. It is a crucial sample preparation step for liquid and gas chromatography. Typical applications include the analysis of pesticide and various pollutants in water, drug in food and blood, as well as oil and grease in environmental samples.
The working principle behind SPE is the adsoprtion of analytes to the solid phase (sorbent material in SPE cartridge), thus removing them from the original liquid phase (sample fluid). The type of SPE cartridge has to be selected based on the chemical and physical properties of the analytes.
Why automate SPE?
Solid phase extraction is a time consuming procedure that requires careful and controlled handling. Performing SPE on large sample batches can be strenuous and prone to human error. In fact, majority of the time spent and errors during chemical analysis come from sample preparation. PromoChrom's automated SPE equipment can alleviate this problem by executing the steps unattended and with high repeatability and reproducibility. The open design makes it easy to place samples, solvents, cartridges and collection vials. Once that is done, all the user has to do is input the desired SPE steps and hit start. The simple interface allows users to choose which sample and solvent to use, as well as the flow rate and volume. To see all features, take a look at our automated offline SPE and automated online SPE pages.
HPLC (High Performance Liquid Chromatography)
If I am not sure which column to use and I cannot afford many columns, which column should I start with?
The most popular column is a C18 (or ODS) column. The column should be able to cover a wide application range, easy to give good result, and has a reasonable price. One example is the PCTsil C18 column Find out more about a versatile HPLC column.
What are the basic components for a HPLC system?
A workable HPLC system must include a sampler, a pump, a column, a detector, and data processor (computer with software). An degasser and a column oven may also be used for better quality of analysis. Basically there are two types of designs. One is integrated design which build all the components into one box. The other type is modular design which allows swap and change of components after installation.
There are so many brands of HPLC available in the market, how do I judge which one is the best for me?
Although the specification and price are important, they should not be the only standard. For many chemists, the meaning of the specification may not be easy to understand. Sometimes the sales person may mislead you using their specification. Here are some other items you may consider: a) feedback from other users, b) hands-on experience or an instrument demo, c) support capability of the vendor, d) cost of maintenance, e) possibility of future upgrade.
What are the important specifications for a sampler?
Reproducibility, range of injection volume, linearity over the injection range, and carry over. A good linearity is very helpful when your sample concentration varies a lot or when you want to make a calibration curve using only one or two standard solutions. The carry over property is very critical in trace analysis. Sometimes a very complicated washing program has to be used before each sample run to avoid carry over problem.
What are the differences between a high pressure gradient pump and a low pressure gradient pump?
In a low pressure gradient pump, different mobile phase is mixed using a valve before entering the pump head. In a high pressure gradient pump, each different mobile phase is delivered by an individual pump head and then mixed at the pump outlet. The low-pressure gradient design is of lower cost as it only use one pump head. It can also use more types of mobile phase without significant increase of cost. The high-pressure gradient design provides a much faster gradient since solvent mixing point is much closer to the column head. This is measured using delay volume. The value can be 50-300 uL for high pressure gradient pump and can be 2 to 3 times larger for a low pressure gradient pump. A small delay volume is important when the analysis time is short or the flow rate is low. If the delay volume is too large, it become impossible to obtain reproducible gradient run since the planed composition cannot reach the column head before a run is finished..
What are the important specifications for an isocratic pump?
Maximum working pressure, flow rate range, flow reproducibility and accuracy, pressure pulsation, pressure monitoring and over pressure shut-off function.
What are the important specifications for a gradient pump?
Maximum working pressure, flow rate range, flow reproducibility and accuracy, gradient reproducibility and accuracy, delay volume, pressure pulsation, pressure monitoring and over pressure shut-off function.
Why is an on-line degasser always recommended for a gradient pump?
Permanent gas has different solubility in different solvents. When different solvents are mixed on line, the dissolved gas can be released from solvent due to changed solubility. To verify this, you may add some water to methanol and observe the large amount of bubbles released from methanol. The bubble formed can cause higher baseline nose and shift of retention time. If you are doing an isocratic LC, a degasser is not so critical since most bubble has been removed when you filter the mobile phase under vacuum.
How many types of detectors are available for HPLC?
UV detector, fluorescence detector, electrochemical detector, conductivity detector, refractive index detector, evaporative light scattering detector, chiral detector, radioactive detector, mass spectrometry detector (MSD).
How to select a suitable detector according to my application?
If your compounds absorb UV light within the range above 200 nm, a UV type detector could be the best choice. It is the most robust and most widely used HPLC detector. Fluorescence detector can have 10 to 100 times better sensitivity for some compounds of large aromatic ring in the structure (such as poly aromatic hydrocarbons). Electrochemical detector is useful for compounds that can be easily electrochemically reduced or oxidized, such as phenols, aromatic amines, and some carbohydrates. It can produce better method sensitivity for such compounds than a UV detector due to better selectivity. However, as the electrodes have direct contact with column effluent and involve reaction with sample matrix, the response may not be as stable as a UV detector. Conductivity detector is mainly for inorganic ions in an ion exchange based liquid chromatographic system. Normally a suppressor is needed to remove background ions from mobile phase for a satisfactory detection sensitivity. Refractive index detector can detect any compounds but the sensitivity is around 100-1000 times lower than a UV detector. Another limit of this detector is that it cannot do gradient LC. This detector is mainly used for organic compounds that do not have reasonable UV absorbance, such as small organic acids, carbohydrates, and some polymers. Evaporative light scattering detector is of similar application range as refractive index detector and it can allow gradient HPLC. The disadvantage of this detector is consumption of large amount of nitrogen for evaporating the mobile phase. MSD is getting more and more popular although the price is 10-20 times higher than other HPLC detectors. It can act as a general purpose detector using scan mode or as an highly selective detector using selective ion monitoring. The most important advantage of MSD is in identity confirmation.
How to choose the flow cell volume for a UV detector?
If the flow cell is too large, two adjacent peaks may get mixed in the cell. If it is too small, the noise may be higher due to less light reaching the photo diodes. The higher back pressure could also limit the flow rate range. The peak size may be used for the selection. A narrow peak need a smaller flow cell. The rule of thumb is the flow cell volume should not be more than 1/3 of the peak volume. For example, if the peak width is 0.1 min at 1 mL flow rate, the flow cell volume should not be more than 33 uL. If your flow rate is 0.01 mL/min, the flow cell volume should be around 0.3 uL.
How to choose an equivalent C18 column for my HPLC?
The situation in HPLC column is quite different from GC columns. C18 or ODS columns from different supplier can give very different elution profile. This is because the silica properties, the carbon coverage and the surface area varies considerably. If you want to be sure of the same elution pattern without changing the mobile phase, the column from the same manufacturer should be used. If you want to try a column of similar properties, you can look at columns that have similar surface area and carbon loading for the packing material.
How to choose a guard column?
Its volume should not be more than 10% of the analytical column. The packing should be similar to the one in the analytical column. If this is not available, you may use a guard column which has weaker retention to your analytes than the analytical column.
What are the important specifications for a column?
Separation efficiency, inertness, durability, pH range, and batch to batch reproducibility.
How to choose a chiral column?
It is difficult to predict if a chiral column will give good separation to a pair of chiral isomers. So the best way is to search literature to see if it has been done before. Or you may send your sample to the column supplier for a sample testing.
Some columns are end capped. What is it good for?
When the silica is bonded with C18, or C8, there are still some active sites on the silica left over. An end capping using a more active short chain can make the column more inert to some basic compounds and make the column more resistant to basic dissolution to the silica material. However such capping is not stable at low pH (< 3). So normally such column is not very suitable for mobile phase of low pH.
How to make a column last longer?
Filter your sample and mobile phase. Make sure the pH of the mobile phase is within the working range of the column. Flush the column with methanol or acetonitrile if it is not going to be used for sometime.
Why are acetonitrile and methanol most commonly used solvent for reverse phase HPLC?
These two solvents can mix well with water at any ratio and do not have significant UV absorption within the UV detection range. They are also easy to obtain.
What are the differences between acetonitrile and methanol as mobile phase?
Acetonitrile has lower UV absorbance at short UV region (200-210 nm) and is better choice if your UV detection is in this region (such as for some small organic acids). Acetonitrile also generate lower back pressure than methanol. This is good when you are trying to use higher flow rate or when your column is partially clogged. On the other hand, methanol is of lower cost. It is also more stable than acetonitrile. When acetonitrile is dry and is exposed to ambient light, some polymers may be produced which may block your HPLC valves or filters. It is a good idea to use amber bottles for acetonitrile.
What are the differences between reverse phase HPLC and normal phase HPLC?
In the reverse phase LC, the mobile phase is polar and the column packing is non-polar. So compounds of high polarity will elute faster than compounds of low polarity. In case of normal phase HPLC the order is opposite. The column packing is polar and the mobile phase is of low polarity. Compounds of low polarity will elute faster. Nowadays most people use reverse phase LC due to its better reproducibility and ease in solvent handling. Normal phase LC is easier to have retention time shift due to moisture build up in the silica packing. Normally a gradient elution cannot be used for normal phase LC. However normal phase LC do have its advantages. It is good for separation of isomers and compounds of very low polarity. Normally reverse phase LC is not good for such situations.
Which parameter can tell me about the instrument status?
The pump pressure reflects the status of the column and system. A very low pressure (e.g. < 10 bar) indicates leak in the system. A very high pressure tells some block in the column or tubing. If the pressure fluctuate widely (e.g. change from 10 bar to above 50 bar), the pump inlet may have been blocked, some bubble could be in the system, or the inlet valve is malfunctioning. It is a good habit to keep an eye on the pressure while the instrument is running.
How do I add soak time on the automated SPE system?
1. Add a new method step
2. Select "Wait" under "Action"
3. Select "Timed" under "Type" and set the number of minutes.
The run will wait out the specified duration when reaching this step.
Does the product load the exact sample volume?
It is always recommended to load the entire sample, this can be done by setting the volume to be 2-5% more than the anticipated sample volume.
Can the SPE system automate sample bottle rinsing?
Yes, the SPE-03 system can automate sample bottle rinsing if equipped with MOD-004 or MOD-00P. MOD-004 can be used for up to 250mL bottles and MOD-00P can be used for up to 1L bottles.
What types of SPE cartridges are compatible with you automated SPE system?
1/3/6mL SPE cartridges are compatible by default on the SPE-03 system. Customized adapters can be made for up to 70mL cartridges.
Can I program the system to pause during run?
1. Add a new method step
2. Select "Wait" under "Action"
3. Select "Manual Resume" under "Type"
The run will pause when reaching this step. To resume, tap "start".
Does the automated SPE system require pressurized air/nitrogen?
Pressurized air/nitrogen should be connected to the system if SPE cartridge drying is required. The system comes with 1.5m of 1/4" OD or 6mm OD air tubing.
What power supply does your automated SPE system run on?
PromoChrom's automated SPE systems can run on 120Vac or 240Vac.
Does the automated SPE system need to be in a fume hood
PromoChrom's automated SPE systems can be placed in a fume hood or out on an open lab bench as long as adequate ventilation is provided. Fume hood is recommended when using strong solvents such as DCM.
How much bench space is required for the SPE-03?
The SPE-03 system on its own has a 34cm by 34cm footprint. However, we recommend a 80cm wide by 50cm deep open space to allow enough room for sample bottles on each side.
How are sample and solvent wastes collected?
The SPE-03 and SPE-04 systems have separate waste outlets for sample and solvent waste. Waste containers should be placed below the SPE-03 bench to allow for draining by gravity.
How do I go about ordering?
Contact us to get a quotation with pricing and lead time. Our experts will help answer any techinical questions and recommend the necessary features based on your application. Once payment (private labs) or PO (government labs) is received, we will start working on your order and have it shipped within the specified lead time.
Is shipping included in my order?
Shipping is included in the price of all our SPE instruments. For accessories and consumables, free shipping is included in orders over $500. Customs clearance and any custom fees/taxes are handled by the buyer.
Can I request a product demo?
We offer two types of demos:
1. Virtual demo through Zoom and Teams at no cost. You will be able to see the product run and ask any questions regarding its operation.
2. On-site demo with a brand new system customized to your needs with 30 days evaluation and option to extend. Any demo payments count towards the purchase price.
Contact us for a virtual or on-site demo
What is included in a product demo?
The on-site product demo includes a brand new system customized to your application, free shipping, technical support and 30 days evaluation after the product is installed.
How do I improve recovery on a new method?
1. Ensure that adequate flow rate is used. If the method does not have a predefined flow rate, try 5mL/min for pre-conditioning and sample loading. Final elution can be set to 3mL/min.
2. Check that nitrogen supply is turned on and effectively drying the SPE cartridges. Start with 20psi and 2.0L/min. Increasing the drying time or nitrogen pressure and flow rate will improve drying.
3. Add 1min soak time to pre-conditioning and final elution steps.
4. Reduce the final elution flow rate to 3mL/min
5. Ensure samples are prepared correctly, i.e. adding the correct solvents and preservatives and pH adjustment
6. Check for any contamination or expiration in the standards and preservatives
How can I reduce the amount of water in the fractions?
There are 3 possible adjustments:
1. Drying time - can be directly changed from the interface
2. Nitrogen pressure - increase nitrogen from 20psi to 30psi on the pressure regulator
3. Flow rate - turn the flow meter knob to increase flow rate to 2.5L/min or more if necessary
Can I run the method before the samples are loaded?
Yes, this can be done by adding a "Wait" step after the pre-conditioning steps. The system will pause automatically after pre-conditioning is done. Once the samples are loaded, tap "start" to resume extraction.
How do I find the source of background contamination
Background contamination can come from many different sources in the lab including containers, standards, solvents, SPE equipment, GC/LC, pressurized nitrogen and even the surrounding air. To check our automated SPE for background, run a "Clean" step followed by "Collect" using your elution solvent and analyze the fractions. This will check all the system lines for any traces of contamination. Solvents and standards should also be analyzed on the instrument. If no contamination is detected, perform the extraction procedure again while making sure each manual step is performed with care.