SPE-04   automated online SPE system
Higher sensitivity. Faster. Increased column life.
Taking sample preparation to the next level
- Integration with LC/LC-MS
- Online derivatization
- Two-tier online SPE  Learn More
The online SPE system not only performs automated solid phase extraction, it also delivers the cleaned up samples directly to your LC/LC-MS for analysis. Using just a simple cable and tubing connection, the SPE-04 can synchronize its operations with the LC/LC-MS to prepare and analyze samples in parallel.
All-in-one SPE solution
SPE-04 is a flexible and versatile platform for automated online solid phase extraction. The full system is capable of :
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Works with any HPLC that has remote input
User friendly software simplifies operation and integration with HPLC
Does not interfere with LC-MS software
|Sample Capacity||18, 26 or 38 sequential|
|Sample Volume||0.5 to 4/8/20 mL depending on sample container|
|Flow Rate||1 to 30 mL/min|
|Dimensions||34 cm x 42 cm x 35 cm|
|Applications||Forensic drugs, biological samples and pesticide residue|
|Special Features||Computer not required in offline mode, dedicated SPE column for each sample, small footprint|
|SPE-04-01||Offline SPE model|
|SPE-04-02||Online SPE model, with sample injection for HPLC|
|SPE-04-03||Online SPE model, with sample injection for HPLC and online derivatization module|
|SPE-04-04||Online SPE model, SPE-04-03 with two-tier online SPE|
Setup and Operation
SPE-04 online SPE can be integrated with HPLC by connecting to the HPLC's remote trigger and sample inlet using our provided cable and tubing.
The simple push-button interface on the system can store up to 3 different methods and is sufficient for most applications. To store and access more methods, users can use our computer software.
Example : Setup for direct analysis of polycyclic aromatic hydrocarbons (PAH) in tap water at ppt level using PromoChrom's online SPE (dotted line area) coupled with HPLC :
The HPLC system includes an Agilent G1310A isocratic pump and a G1314A variable wavelength detector. Since the online SPE takes care of sample loading and injection, the HPLC only needs a pump, a detector, and a computer with the ChemStation software.
Through specially designed valves and syringe pumps, the online SPE transfers samples to the SPE column and then to the HPLC system. Users only need to select the necessary actions, such as “load sample”, “add reagent 1”, “inject”, “start LC”, etc.
Our device uses a remote signal to trigger the start of HPLC. While the HPLC is running a sample, the online SPE can start extracting the next sample. This overlapped introduction reduces sample preparation time.
Two-tier Online SPE
From hours down to minutes. PPB detection limit with HPLC.Application Note
Integration of solid phase extraction or column cleanup with chromatographic analysis may take two approaches :
- Direct Coupling - sample loaded in cleanup column is completely transferred to analytical column using a switching valve
- Indirect Coupling - sample treated like in the case of standalone SPE. Portion of collected fraction is then injected into the analytical column using an autosampler
|Direct Approach||Significantly higher sensitivity and faster throughput||SPE to analytical column compatibility issues.
Difficult to regenerate SPE column, a hassle to change online SPE column installed on high pressure switching valve
|Indirect Approach||Decoupled from HPLC column, small injection volume, therefore no compatibility issues.|
No online SPE column to replace.
Much more time consuming
To overcome the disadvantages of the two online SPE approaches while maintaining their advantages, PromoChrom developed the two-tier online SPE solution.
- Samples are first processed like in the case of standalone SPE
- The collected fraction is then modified using a solvent and then transferred to a second online SPE column on a switching valve
The first SPE treatment removes most of the interference. The second SPE column is used for enrichment and further cleanup. Since most of the particulates are trapped by the first SPE column, the lifetime of the second (online) column is considerably extended. This reduces the need to change out the online SPE column. Other benefits include :
- Reduced peak broadening
- Reduced background noise
- Large volume sample introduction - achieving ppb detection limit for analysis of PAH in water using HPLC UV detector