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Protein Purification

Protein Purification

Automated High Throughput Purification System for

Proteins and other Biomolecules

  • Antibody Purification

  • Antigen and Enzyme Extraction

  • Metabolite Profiling

  • Oligonucleotide Purification

Up to 40X faster than manual protein purification

SPE-03 System for Protein Purification

SPE-03 - automated Protein Purification System

Automated purification of 8 samples in parallel, up to 1L samples,
using as many as 9 buffer solutions.

Compatible with 1mL to 70mL columns
eg. GE HiTrapTM, gravity-flow columns and standard SPE cartridges.

Positive-pressure liquid delivery ensures consistent flow rate and fixed process time for each protein purification batch. Supervision is no longer required for gravity-flow columns, all samples start and finish simultaneously. Controlled flow also allows for increased column packing capacity for even higher sample throughput.

Up to 2 fractions per sample enabling targeted automated extraction of biomolecules and method development. More fractions can be collected by programming pause steps and switching out fraction tubes.

Automated column regeneration possible depending on application.

Customizations available upon request.

What does our customer say about SPE-03 System for automating protein purification?

The AlphaThera team shared data on their use of SPE-03 system for protein purification

SPE-03 protein purification in AlphaThera

SPE-03 at AlphaThera Lab


"With the SPE-03, we can run purification overnight, so it saves us a day, improves consistency, and removes one of the more redundant, time-consuming tasks in our workflow."

Overall feedback from AlphaThera team

  • High production level with low batch-to-batch variability (See Figure 1)

  • Significant time savings, with total purification time being reduced from 2 work days to just overnight (See Figure 2)

  • Easy scale up, allowing a 5x scale up in production to be easily accommodated (See Figure 3)

  • Easy to maintain the instrument

AlphaThera -feedback of SPE-03

AlphaThera is a biotechnology company focused on simplifying and standardizing antibody labeling to enable confident bioconjugation reactions. To optimize their protein purification process and enhance efficiency, the company deployed PromoChrom's SPE-03 automated SPE system. They also returned to buy a second system just months of installation five times.


AlphaThera has seen tremendous improvements in their production levels, batch-to-batch consistency, and purification time with the use of SPE-03. They were also able to scale up production by five times while keeping instrument maintenance simple.


Highly Inert and Corrosion Resistant

Compatible with biological samples, acids, bases and salts


Operates under temperatures low as


For proteins such as enzymes and antigens that require low temperature preservation


Easily Programmable Touch Interface

Saves up to 100 methods for automated protein purification, system cleaning and column regeneration

Dual-column Feature on the SPE-03

Automated Protein Purification Steps:

Choose from up to 9 buffers to condition columns


Elute columns using selected buffer(s). Can collect up to 2 separate fractions automatically.

Wash Column

Choose from up to 9 buffers to remove impurities

Regenerate Column

Wash and regenerate columns with buffer(s) and preserving agent(s)

Clean System

Choose from up to 9 buffers to clean sample and column lines

Condition Column
Load Sample

Deliver up to 1L of samples such as cell lysate or other biological fluids through columns

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