Automated High Throughput Purification of
Proteins and other Biomolecules
Antigen and Enzyme Extraction
Up to 40X faster than manual protein purification
SPE-03 8-Channel System
Automated purification of 8 samples in parallel, up to 1L samples,
using as many as 9 buffer solutions.
Compatible with 1mL to 70mL columns
eg. GE HiTrapTM, gravity-flow columns and standard SPE cartridges.
Positive-pressure liquid delivery ensures consistent flow rate and fixed process time for each protein purification batch. Supervision is no longer required for gravity-flow columns, all samples start and finish simultaneously. Controlled flow also allows for increased column packing capacity for even higher sample throughput.
Up to 2 fractions per sample enabling targeted automated extraction of biomolecules and method development. More fractions can be collected by programming pause steps and switching out fraction tubes.
Automated column regeneration possible depending on application.
Customizations available upon request.
What does our customer say about SPE-03 for protein purification?
The AlphaThera team shared data on their use of SPE-03 for protein purification
SPE-03 at AlphaThera Lab
"With the SPE-03, we can run purification overnight, so it saves us a day, improves consistency, and removes one of the more redundant, time-consuming tasks in our workflow."
Overall feedback from AlphaThera team
High production level with low batch-to-batch variability (See Figure 1)
Significant time savings, with total purification time being reduced from 2 work days to just overnight (See Figure 2)
Easy scale up, allowing a 5x scale up in production to be easily accommodated (See Figure 3)
Easy to maintain the instrument
AlphaThera is a biotechnology company focused on simplifying and standardizing antibody labeling to enable confident bioconjugation reactions. To optimize their protein purification process and enhance efficiency, the company deployed PromoChrom's SPE-03 automated SPE system. They also returned to buy a second system just months of installation five times.
AlphaThera has seen tremendous improvements in their production levels, batch-to-batch consistency, and purification time with the use of SPE-03. They were also able to scale up production by five times while keeping instrument maintenance simple.
Highly Inert and Corrosion Resistant
Compatible with biological samples, acids, bases and salts
Operates under temperatures low as
For proteins such as enzymes and antigens that require low temperature preservation
Easily Programmable Touch Interface
Saves up to 100 methods for automated protein purification, system cleaning and column regeneration
Dual-column Feature on the SPE-03
Automated Protein Purification Steps:
Choose from up to 9 buffers to condition columns
Elute columns using selected buffer(s). Can collect up to 2 separate fractions automatically.
Choose from up to 9 buffers to remove impurities
Wash and regenerate columns with buffer(s) and preserving agent(s)
Choose from up to 9 buffers to clean sample and column lines
Deliver up to 1L of samples such as cell lysate or other biological fluids through columns